Figure 5.

Changes in the transcript levels of LsNCED and LsABA8ox during incubation under different conditions. A, Expression levels of LsNCED and LsABA8ox after light and GA₁ treatment. The expression levels of these genes were analyzed by QRT-PCR as described in the text. 0 h indicates seeds imbibed for 3 h in the dark (just before light treatment). FR+GA₁ indicates seeds treated with FR and 1 mm GA₁. The results were normalized to the expression of 18S rRNA (internal control), and the expression levels of all genes examined are given relative to a reference value, the transcript level of LsNCED4 at 0 h, set to 1. Two independent experiments were performed, and averages are shown with ses. B, Expression levels of LsNCED and LsABA8ox under photoreversible conditions 3 h after light treatment. The results were normalized to the expression of 18S rRNA, and then the highest value of each gene was set equal to 1. Two independent experiments were performed, and averages are shown with ses.