Figure 2.

N. attenuata plants of the AZ accession have lower levels of M. sexta OS-elicited SIPK activity than do plants of the UT accession. N. attenuata UT and AZ plants were grown under identical conditions. Rosette-stage leaves (+1 position) were wounded with a pattern wheel; 20 μL of W+W or W+OS was applied to the wounds. Leaves from five replicate plants were harvested at the indicated times after elicitation and pooled. Kinase activity was analyzed by an in-gel kinase assay using myelin basic protein as the substrate.