Figure 6.

N. attenuata UT and AZ accessions produce different amounts of secondary metabolites that function as direct and indirect defenses. N. attenuata UT and AZ plants were grown under identical conditions. A to C, Rosette-stage leaves (+1 position) were wounded with a pattern wheel; 20 μL of either W+W or W+OS was applied to the wounds. Nontreated plants served as control. Four days after treatments, plant tissue samples were harvested; nicotine (A), chlorogenic acid (B), and rutin (C) concentrations were analyzed using HPLC. The emission of CAB was measured in treated leaves (+2 position) at 24 to 32 h posttreatment with either W+W or W+OS, with no treatment as control (D), or 150 μg MeJA in lanolin paste, with lanolin as control (E). Absolute emission is not comparable between D and E as these were two separate experiments (unpaired t test; *, P < 0.05; **, P < 0.01; ***, P < 0.001, n = 5).