Table I.
Properties of recombinant poplar KTIsa
| KTI | Size (Amino Acid) | Predicted pI | Molecular Weight
|
Protein Solubilityb
|
||
|---|---|---|---|---|---|---|
| Predicted | Actual (SDS-PAGE) | Distilled, Deionized Water | 50 mm Tris (pH Range) | |||
| kD | ||||||
| TI2 | 213 | 4.42 | 23.1 | 24 | Insoluble | 2 and 5–12 |
| TI3 | 202 | 6.15 | 22.0 | 26 | Soluble | 2–4 and 9–12 |
| TI4 | 212 | 4.96 | 23.1 | 24 | Insoluble | 2–3 and 8–12 |
| TI5 | 214 | 3.98 | 22.9 | 30 | Insoluble | 7–12 |
| TI6 | 202 | 5.32 | 21.8 | 23 | Partially soluble | 2–3 and 7–12 |
a
Predicted Mr and pI were calculated by Vector NTI Advance 9 software; SDS-PAGE-determined Mr was extrapolated from the logarithmic-transformed migration distance of the proteins compared with protein standards (low range; Bio-Rad).
b
Recombinant protein solubility was assessed by dialysis of proteins in water and 50 mm Tris buffers of pH 2 to pH 12. For solubility in Tris buffer, the pH range for which the proteins were soluble is shown.