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Freezing tolerance of M. falcata (MF) and M. truncatula (MT) plants after 4 weeks growth at 20°C/18°C day/night (NA), or after an additional 4 weeks at +2°C (CA). A, Crown regions used for electrolyte leakage and whole-plant freezing assays. B, Temperature at which 50% of the plants failed to regrow (LT50) after freezing to the indicated temperatures. C, Temperature at which 50% electrolyte leakage (TEL50) occurred after freezing.
