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. 2008 Mar;146(3):904–915. doi: 10.1104/pp.107.109264

Figure 1.

Figure 1.

Silencing JAR4 and JAR6 in irjar4/6 plants. A, Mean (±se) of JA-Ile/Leu and JA concentrations in four replicate +1 leaves (one leaf position older than the source-sink transition leaf) of wild-type and irjar4/6 (irjar4-2/6-2, irjar6-1/4-1) plants, as well as of plants independently silenced for JAR4 or JAR6 (inset; irjar4-1, irjar4-2, irjar6-1, irjar6-2) 1 h after JA treatment. B, Accumulation of JAR4 or JAR6 transcripts in irjar4/6 and wild-type plants. RNA was isolated from 10 leaves growing at node +1 of separate irjar4/6 plants or five pooled +1 leaves of wild-type plants. Leaves were harvested 1 h (for irjar6-1/4-1 and wild-type control) or 3 h (for irjar4-2/6-2 and wild-type control) after OS treatment. A duplicate gel stained with ethidium bromide was used as an RNA-loading control.