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. 2008 Mar;146(3):904–915. doi: 10.1104/pp.107.109264

Figure 2.

Figure 2.

Impaired direct defenses and resistance of irjar4/6 plant to M. sexta attack could be restored by treatment with JA-Ile but not JA-Leu or JA-Val. A, Mean (±se) levels of TPIs and nicotine in four replicate leaves growing at the +1 node of wild-type and two lines of irjar4/6 plants (irjar4-2/6-2 and irjar6-1/4-1), as well as plants independently silenced for JAR4 or JAR6 (inset; irjar4-1, irjar4-2, irjar6-1, irjar6-2). Rosette-stage plants were wounded and treated with 0.25 μmol JA (W + JA), JA-Ile (W + JA-Ile), JA-Val plus JA-Leu (W + JA-Val + JA-Leu), or water (W + W). Ninety-six hours after treatment, treated leaves were harvested for analysis. Asterisks represent significant difference between members of a pair (Student's t test; *, P < 0.05; **, P < 0.01; ***, P < 0.001). B, Mean (±se) mass of 18 replicated M. sexta larvae after 9 d of feeding on wild-type and two lines of irjar4/6 plants (irjar4-2/6-2 and irjar6-1/4-1). Rosette-stage plants were wounded and treated with 0.25 μmol JA-Ile (W + JA-Ile), JA-Val (W + JA-Val), JA-Leu (W + JA-Leu), or water (W + W). One day later, one freshly hatched larva was placed on treated leaves of each treated plant. Asterisks represent significant difference between members of a pair (Student's t test; *, P < 0.05; **, P < 0.01; ***, P < 0.001).