Figure 3.

Silencing both JAR4 and JAR6 suppresses OS-elicited transcriptional response, which can be restored by JA-Ile supplementation. Leaves at node +1 of rosette-stage wild-type and irjar4/6 (irjar4-2/6-2) plants were wounded and treated with OS or 0.25 μmol JA-Ile. After 180 min, leaf material was harvested and RNA was extracted, transcribed into cDNA, and labeled with fluorescent dye (wild-type samples, Cy3; irjar4/6 samples, Cy5). For each treatment, three pools of wild-type samples and three pools of irjar4/6 samples were made, each consisting of three biological replicates. Each microarray was hybridized with one wild-type pool and one irjar4/6 pool of labeled cDNA. Three microarrays per treatment were hybridized and statistically analyzed. In the OS treatment (left bars), genes significantly (P < 0.05) higher (Cy3/Cy5 > 1.5) and lower (Cy3/Cy5 < −1.5) than those expressed in irjar4/6 plants are shown with red and green bars, respectively. In the JA-Ile treatment (right bars), genes not differently expressed (−1.5 < Cy3/Cy5 < 1.5) in two genotypes are shown with gray bars. Genes are identified by a two-letter genus/species designation followed by the gene name. St, Solanum tuberosum; Nt, Nicotiana tabacum; Ns, Nicotiana sylvestris; Sl, Solanum lycopersicum; Sa, Solanum americanum; AMY, β-amylase; NIA1, nitrate reductase; GPPH, α-glucan phosphorylase, H isozyme; ASN1, Asn synthase; DHYS, dehydroquinate synthase; PhD, prephenate dehydratase; EPSPS, 5-enolpyruvylshikimate-3-P synthase; 4CL, 4-coumarate-CoA ligase.