Figure 5.

Genes differently expressed in aslox3 and irajr4/6 plants. Leaves growing at +1 node of rosette-stage aslox3 and irjar4/6 (irjar4-2/6-2) plants were OS elicited. After 180 min, leaf material was harvested and RNA was extracted, transcribed into cDNA, and labeled with fluorescent dye (aslox3 samples, Cy3; irjar4/6 samples, Cy5). Three pools of aslox3 samples and three pools of irjar4/6 samples were made, each consisting of three biological replicates. Each microarray was hybridized with one aslox3 pool and one irjar4/6 pool of labeled cDNA. Three microarrays per treatment were hybridized and statistically analyzed. Genes significantly (P < 0.05) higher (Cy3/Cy5 > 1.5) and lower (Cy3/Cy5 < −1.5) expressed in aslox3 plants are shown with red and green bars, respectively. Genes are identified by two-letter genus/species designations followed by the gene name. St, Solanum tuberosum; Nt, Nicotiana tabacum; Sl, Solanum lycopersicum; Sa, Solanum americanum.