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. Author manuscript; available in PMC: 2009 Feb 6.
Published in final edited form as: Vaccine. 2007 Dec 26;26(6):786–796. doi: 10.1016/j.vaccine.2007.11.080

Figure 3.

Figure 3

Effect of Nlac PorB on induction of OVA-specific total IgG and IgM in OVA-immunized mice. Groups of five mice were immunized s.c. with 10 µg of OVA alone or dissolved in saline containing 200µg of alum or 10 µg of Nlac PorB every two weeks for a total of three immunizations. Sera were examined prior to the first immunization (week 0) and two weeks after each immunization (week 2, 4 and 6). A) and B) OVA-specific total IgG and IgM antibodies in the sera were measured by ELISA, quantified by using standard IgG and IgM reference curves and expressed in µg/ml. The values represent the mean of triplicate wells for each individual mouse serum ± standard deviation. Significant differences (p = < 0.05) with the OVA group were calculated by Mann Withney test and are indicated by the asterisks.