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. Author manuscript; available in PMC: 2009 Feb 6.
Published in final edited form as: Vaccine. 2007 Dec 26;26(6):786–796. doi: 10.1016/j.vaccine.2007.11.080

Figure 5.

Figure 5

Effect of Nlac PorB on specific and non-specific splenocyte proliferation. Splenocytes from mice immunized with OVA alone, alum/OVA, Nlac PorB/OVA or Nme PorB/OVA were incubated for 72h with OVA (10 µg/ml, black bars) for induction of specific proliferation or with LOS (10 µg/ml, gray bars) and Concanavalin A (ConA, 10 µg/ml, white bars) for induction of non-specific proliferation. Medium alone was used as a negative control. Splenocytes proliferation was determined by the MTT assay. The values represent the mean of quadruplicate wells ± standard deviation and expressed as Stimulation Index (SI) as described in the text. Significant differences (p=<0.05) with the OVA-immunized group were calculated by Mann Withney test and are indicated by (*) for ConA stimulation, (**) for LOS stimulation and (***) for OVA stimulation. No significant differences were detected between the OVA group and the alum/OVA group.