Figure 6.
Nlac PorB stimulates B cells in vitro. Surface upregulation of CD86, MHC II and CD40 was measured by flow cytometry. In all the histograms the isotype control is indicated by the gray area and the medium is indicated by the thin line. B cells from A) C57Bl/6 wild type mice and B) TLR2 knockout mice incubated for 24h with Nlac PorB (10 µg/ml, thick line) and Nme PorB (10 µg/ml, dotted line). B cells from C) wild type mice and D) TLR2 knockout mice incubated for 24h with Pam2CSK4 (100 ng/ml, dotted line), Pam3CSK4 (100 ng/ml, dashed line) and LOS (100 ng/ml, thick line).