Table 2. Classification of genes showing similar regulation between the IGS and the comparison of CD44+CD24− cells and total LNCaP cells.
| Family | Genes |
|---|---|
| Cytokine | CXCL2, IL3, IL4, PBEF1, SCYE1, TNF |
| Enzyme | ARIH1, ATIC, CHPT1, CPT1A, DNMT3A, DNMT3B, ELP3, ERCC5, GAPDH, ICMT, LDHA, LDHB, PRIM2A, RALBP1, UBE2D2, WWOX |
| G-protein-coupled receptor | ADORA2B, OPRK1 |
| Growth factor | FIGF, NRG1, TDGF1, TGFB1 |
| Ion channel | CFTR, CLCN3 |
| Kinase | CSNK1D, CSNK2A1, ERBB4, ERN1, ETNK1, NUAK2, PGK1, PIM2, WEE1 |
| Peptidase | ADAM10, ADAM19, CASP1, CASP4, CASP8, F7 |
| Phosphatase | NUDT5, PPP1R12C |
| Transcription regulator | BRF1, CDKN2C, CEBPG, CTNNB1, DNMT3L, E2F4, ELF4, ELL2, ETS1, FOS, ISGF3G, KLF10, MYC, NFYB, NKX3-1, RELA, SIM1, XBP1 |
| Translation regulator | EIF4E2 |
| Transmembrane receptor | MSR1, SFRP1, TNFRSF10B |
| Transporter | APOC2, BCAP31, GOPC, NUP37, NUP43, NUP107, NUP133, PLP2, SEC13L1, STX6 |
| Others | AIM1, ARPC2, ARPC4, ARPC5, C4ORF7, C6ORF107, CDC2B, CDKN1B, CNOT4, COL4A2, CSTF1, ELP4, ELP5, ELP6, EMP1, FRAT1, GABARAPL1, HSPA2, HSPE1, IER5, IKBKAP, ISG15, JTV1, ITGB6, KLHL20, MAPT, MLF1, MT1A, NUP85, PLAA, RAD23B, RAD23A, S100A6, SCGN, SERPINB5, SERPINE2, SFTPA1, SNRPN, SWAP70, STATIP1, TAC1, THY1, TOB2, TPD52, VIL2 |
IGS=invasiveness gene signature.
IGS genes that showed a 1.8-fold or greater regulation in the comparison of CD44+CD24− cells vs total LNCaP cells were identified. Of the 68 identified, 42 of them were available in Ingenuity Pathways Analysis databases for analysis of gene function. The functions and the genes identified for those functions are listed.