Figure 5.

Effect of prazosin (PZ) on carboxyfluorescein diacetate succinimidyl ester (CFSE)- and tetramethylrhodamine-5-(and-6)-isothiocyanate (TRITC)-labelled hepatic red blood cells (RBCs) recovered from paracetamol (APAP)-treated mice at 5 h. CFSE-labelled RBCs were administered i.v. to male CD-1 mice 16 h before paracetamol±prazosin treatment. Mice were then given either vehicle control (a), prazosin (35.7 μmol kg⁻¹; b), paracetamol (3.5 mmol kg⁻¹; c) or prazosin 1 h before paracetamol (d) for 5 h. Representative dot plots of RBCs recovered from the livers of treated mice after 5 h are shown. TRITC-labelled RBCs are shown in region 1 with CFSE-labelled RBCs in region 4. RBCs were assayed using fluorescence-activated cell sorting analysis, CFSE-labelled cells were counted until 4000 TRITC-labelled cells had been counted (e). Results are expressed as mean ratio of CFSE-labelled RBCs vs TRITC-labelled RBCs, n=6. ANOVA statistical analysis with Scheffé comparisons was used. ^**P<0.01.