Figure 1.

Dose dependence of lipid mixing of POPG LUV induced by WT and WT-D peptides. Peptide aliquots were added to mixtures of LUV (22 μM phospholipid concentration) containing 0.6% (molar ratio) each of NBD-PE and Rho-PE and unlabeled LUV (88 μM phospholipid concentration) in PBS. The increase of the fluorescence intensity of NBD-PE was measured at 10 min after the addition of the peptide, and the percentage from maximum was plotted versus the peptide/lipid molar ratio. The fluorescence intensity on the addition of Triton X-100 (Triton Biosciences) (0.25% vol/vol) was referred to as 100%. Squares, WT; triangles, WT-D. (Inset) Time profile of the lipid mixing at peptide/lipid ratio of 0.075.