Figure 3.

Proteolytic digestion of membrane-bound NBD-labeled peptides. The fluorescence emission spectra of the NBD-labeled peptide was monitored at 530 nm with the excitation set at 467 nm: (1) addition of 0.1 μM NBD-labeled peptides; (2) addition of 400 μM SUV; and (3) addition of Proteinase-K (50 μg/ml). In A and B, WT was used, and in C, WT-D was used.