Figure 2.

TG-oxytocin (TG-OT) induces HUVEC invasion through Matrigel. The chemoinvasion experiments were performed as described in the Methods section using Matrigel to coat the filters. TG-OT (1 nM) or VEGF (25 ng ml⁻¹) were used as attractants in the lower compartment of a Boyden chamber. The results are expressed as the number of migrating cells per field and are the mean values±s.e.mean of 15 counts from a representative experiment, repeated once with similar results. ^**P<0.001 compared to basal invasion (medium alone). HUVEC, human umbilical vein endothelial cell; VEGF, vascular endothelial growth factor.