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Variation of rate and responses with developmental day. (a) Increase in beating rate, beats per min (b.p.m.) with day of differentiation from d10 to d79 in hESC-CM under basal conditions (r2=0.097, n=60, P<0.02) or after stimulation with 100 nM isoprenaline (r2=0.20, n=60, P<0.001). (b) Change in beating rate in response to isoprenaline (stimulated basal, b.p.m.) (r2=0.15, P<0.002). hESC-CMs, human embryonic stem cell-derived cardiomyocytes.
