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(a) Time course of beating rate, b.p.m. (beats per min) for hESC-CM during 10 min of exposure to isoprenaline (Iso) on different days of development. Examples of sustained response in a d29 culture, and of decaying response in a d20 culture are shown. (b) Relation between desensitization and day of development (rate at 10 min expressed as a percentage of the maximum rate, r2=0.013, P=NS, n=51). hESC-CMs, human embryonic stem cell-derived cardiomyocytes; NS, nonsignificant.
