Figure 2.

Effect of IL-1β on MAPKs and Src kinase activity in primary cortical neurones. Cultures were treated with vehicle (C) or IL-1β (0.3 U ml⁻¹) for 5, 15, 30 or 60 min, and cell lysates were assayed by western blot analysis for (a) ERK, JNK or p38 activation, or (b) Src kinase activation, using specific antibodies against total (t) or phosphorylated (p) isoforms of ERK, JNK, p38 or Src kinase. Images of blots in (a) are from a single experiment representative of three independent experiments carried out on separate cultures. Data in (b) are presented as the mean±s.d. of three independent experiments carried out on separate cultures. ^*P<0.05, ^**P<0.01, IL-1β vs control. ERK, extracellular signal-regulated kinase; IL, interleukin; JNK, c-Jun N-terminal kinase; MAPK, mitogen-activated protein kinase.