Figure 3.

Effect of IL-1RA, PP2 or 3OMS on IL-1β-induced Src kinase in primary cortical neurones. Cultures were treated with vehicle (C) or IL-1β (0.3 U ml⁻¹) for 5, 15, 30 or 60 min, in the absence or presence of (a) IL-1RA (50 ng ml⁻¹), (b) PP2 (10 μM) or (c) 3OMS (15, 25 μM). Cell lysates were assayed by western blot analysis for Src kinase activation using specific antibodies against total or phosphorylated isoforms of Src kinase. Data are presented as the mean±s.d. of three independent experiments carried out on separate cultures. ^*P<0.05, IL-1β vs control, ^#P<0.05, IL-1β+IL-1RA/PP2/3OMS vs IL-1β alone. IL, interleukin; 3OMS, 3-O-methyl-sphingomyelin.