Figure 4.

Effect of IL-1RA, PP2 or 3OMS on IL-1β-induced IL-6 synthesis in primary cortical neurones. Cultures were treated with IL-1β (0.3 U ml⁻¹) for 7 h in the absence or presence of (a) PP2 (10 μM) or IL-1RA (50 ng ml⁻¹), or (b) 3OMS (15, 25 μM). Cell lysates and supernatants were assayed for IL-6 using a specific mouse ELISA. Data are presented as the mean±s.d. of three different experiments carried out on separate cultures. ^**P<0.01, ^***P<0.001, IL-1β vs control, ^#P<0.05, ^##P<0.01, ^###P<0.001, IL-1β+PP2/IL-1RA/3OMS vs IL-1β alone. IL, interleukin; 3OMS, 3-O-methyl-sphingomyelin.