Figure 5.

Cell-cycle distribution and expression of p21/Waf1/Cip1 in SH-SY5Y neuroblastoma cells treated with butyrate (Bu) or VPA. (a) Cell-cycle distribution of HDAC inhibitor-treated neuroblastoma cells. Cells were synchronized by serum deprivation for 16 h before butyrate or VPA treatment (16 h). Values represent percentages of phase-specific populations. MI is the product of G₁/G₂ ratio. (b) RT-PCR analysis of relative mRNA levels after 6 h treatment (left) and western blot analysis (right) of protein expression (30 μg per lane of proteins) after 8 h in the presence of butyrate or VPA (0.9 mM). β-Actin mRNA and protein levels were evaluated as a proof of equal loading. All the experiments on SK-N-BE cells gave similar results.