Fig. 1.

Influence of conditioned medium on transient protein expression. Cells were seeded in culture medium at a density of 0.5 × 10⁶ cells mL⁻¹ with five different ratios of 72-h-old conditioned medium. The experiment was performed in 30 mL shaked cultures with pCEP4-AmCyan as expression vector. AmCyan expression was measured 24, 48 and 72 h post transfection using the signal-to-noise assay. Data (RFU) of one representative experiment are presented (░24 h, ▒48 h, □72 h)