Table 1.
Composition and derivation of plasmid vectors.
| Vector | Parent vector | Promoter | Reading frame** | marker | Stuffer | 3′ UTR |
|---|---|---|---|---|---|---|
| pAJ01 | pPD95.75* | None | 0 | GFP(S65C) | − | Ss era-1 |
| pAJ02 | pPD95.77* | None | 2 | GFP(S65C) | − | Ss era-1 |
| pAJ03 | pPD95.79* | none | 1 | GFP(S65C) | − | Ss era-1 |
| pAJ04 | pPD95.81* | none | S | GFP(S65C) | − | Ss era-1 |
| pAJ08 | pAJ04 | Ss act-2 | S | GFP(S65C) | − | Ss era-1 |
| pAJ09 | pAJ01 | Ss gpa-3 | 0 | GFP(S65C) | − | Ss era-1 |
| pAJ12 | pAJ01 | none | 0 | GFP(S65C) | + | Ss era-1 |
| pAJ13 | pAJ02 | none | 2 | GFP(S65C) | + | Ss era-1 |
| pAJ14 | pAJ03 | none | 1 | GFP(S65C) | + | Ss era-1 |
| pAJ15 | pAJ04 | none | S | GFP(S65C) | + | Ss era-1 |
| PAJ20 | pAJ04 | Ss rps-21 | S | GFP(S65C) | + | Ss era-1 |
| pAJ50 | pAJ08 | Ss act-2 | S | mRFPmars | − | Ss era-1 |
*
See 1995 Fire kit documentation http://www.addgene.org/ for plasmid details.
**
Frames 0, 1 and 2 are the possible reading frames relative to GFP. Frame S is identical to frame 1 except for the removal of the TAG codon, restoring Hind III, Sph I, Pst I and Sal I fusion junctions.