Figure 6.

DNase I footprint of PIC2. The 136-bp substrate with 5′ label either in the strand containing the (6–4) photoproduct (top strand) or in the complementary strand (bottom strand) was incubated with the five repair factors (no XPF⋅ERCC1) and digested with DNase I, and then bound and unbound fractions were separated on nondenaturing polyacrylamide gels; DNA was eluted from the free and bound fractions and analyzed on 6% denaturing polyacrylamide gels. (A) Footprinting gels along with G+A sequence ladder. Solid and open arrows show the site of (6–4) photoproduct. Brackets and arrow indicate protected region and prominent hypersensitive sites. (B) Schematic illustration of footprint of human excinuclease indicating incision sites and protected region.