Figure 3.

JNK activation in TNF-resistant cell lines. TNF-α-sensitive cell lines can activate JNK in response to TNF-α. In T24 and SNU719 cells, JNK is activated in response to TNF-α, whereas DU145 and AGS cells do not show this induction. (A) To examine the activation of JNK, protein extracts from the indicated cell lines were incubated for 6 h with TNF-α (10 ng/ml) and subjected to immunoblotting with phospho-specific and total JNK antibodies. P- and T- indicate phosphorylated proteins and total proteins, respectively. (B) Expression was quantified by densitometric scan of the blots (A). Values are the ratio of p-JNK and t-JNK and are presented relative to each control.