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. 2007 Dec 21;6:81. doi: 10.1186/1476-4598-6-81

Figure 4.

Figure 4

Use of shRNA constructs to knock-down Fra-1 confirm Fra-1-dependent CD44 expression in MMs. A. Western blots of SV40- (MM1) and SV40+ (MM3) lines showing empty vector (EV) controls or RNAi shFra-1 constructs. B. Immunofluorescence image showing CD44 (red) levels MM cell lines. C. Agarose gels on an SV40- cell line showing the efficiency of the RNAi constructs (shFra-1 and shCD44) on knockdown of CD44 and Fra-1 measured by a semi-quantitative RT-PCR reaction and using GAPDH as a control.