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. 1998 Jun 23;95(13):7293–7298. doi: 10.1073/pnas.95.13.7293

Figure 1.

Figure 1

Histidine-FLAG-tagged α1-AT variants. (A) Diagram of α1-AT variants including native α1-AT, α1-PIT/hf, and α1-PDX/hf. The His and FLAG sequences that replace the signal sequence as well as the amino acid substitutions within the RSL are shown. (B) RP-HPLC analysis of purified α1-PDX/hf. The chromatogram shows a single prominent A280 peak eluting at 58 min corresponding to purified α1-PDX/hf. (C) Electrospray MS of purified α1-PDX/hf. The measured molecular mass of α1-PDX/hf and α1-PIT/hf (46,719 ± 3 Da and 46,631 ± 3 Da, respectively) was in close agreement (relative error less than 0.02%) with the calculated values for α1-PDX/hf (46,727 Da) and α1-PIT/hf (46,642 Da).