Table 2.
Identification and analysis of secreted proteins from Trichostrongylus vitrinus ESTs.
| Number | EST sequence ID | Seq Length (aa) | Start | Signal Peptide length (aa) | Description (top hit from non-redundant protein database) | E-value | % Identity (aa) | RNAi phenotype in C. elegans |
| 1 | TvmContig8 | 102 | - | 19 | No significant hits | - | - | - |
| 2 | TVmContig11 | 148 | - | 24 | No significant hits | - | - | - |
| 3 | TVmContig20 | 121 | - | 25 | 24 kDa excretory/secretory protein [Haemonchus contortus] | 3.00E-028 | 63/109 (57%) | |
| 4 | TvmContig28 | 167 | - | 19 | No significant hits | - | - | - |
| 5 | TVmContig35 | 120 | M | 17 | 30 kDa antigenic glycoprotein precursor [Trichostrongylus colubriformis] | 4.00E-006 | 27/65 (41%) | No observed phenotype is found. |
| 6 | TVmContig39 | 208 | - | 18 | Weakly similar to PhosphoDiEsterase family member (pde-2) [Caenorhabditis elegans] | 4.00E-028 | 22/72 (30%) | No observed phenotype is found. |
| 7 | TvmContig68 | 131 | - | 26 | Weakly similar to SaPosin-like Protein family member (spp-19) [Caenorhabditis elegans] | 0.016 | 23/82 (28%) | No observed phenotype is found. |
| 8 | TVmContig75 | 239 | - | 26 | Weakly similar to Na/Ca eXchangers family member (ncx-4) [Caenorhabditis elegans] | 0.3 | 15/39 (38%) | No observed phenotype is found. |
| 9 | TVmContig96 | 120 | - | 15 | Non-ATPase-like family member (rpn-1) [Caenorhabditis elegans] | 2.00E-047 | 94/119 (78%) | EMB, LET embryonic_lethal, maternal_sterile, sick (Sck) |
| 10 | TvmContig101 | 156 | M | 18 | Zinc metalloproteinase nas-13 precursor (Nematode astacin 13) [Caenorhabditis elegans] | 2.00E-008 | 30/96 (31%) | No observed phenotype is found. |
| 11 | TVmContig107 | 85 | - | 27 | No significant hits | - | - | - |
| 12 | TVmContig134 | 190 | - | 26 | No significant hits | - | - | - |
| 13 | TvmContig135 | 116 | - | 19 | Late Embryo Abundant (LEA) related family member (lea-1) [Caenorhabditis elegans] | 4.00E-004 | 22/86 (25%) | No observed phenotype is found. |
| 14 | TVmContig150 | 137 | - | 24 | No significant hits | - | - | - |
| 15 | TVmContig164 | 122 | - | 20 | Weakly similar to Human xnp gene related protein 1 [Caenorhabditis elegans] | 0.18 | 18/57 (31%) | None |
| 16 | TVmContig170 | 85 | M | 19 | No significant hits | - | - | - |
| 17 | TVmContig171 | 131 | M | 15 | No significant hits | - | - | - |
| 18 | TVmContig172 | 129 | - | 26 | Weakly similar to abnormal NUClease family member (nuc-1) [Caenorhabditis elegans] | 0.016 | 12/30 (40%) | life span abnormal (Age) |
| 19 | TvmContig178 | 146 | - | 17 | Weakly similar to Maternal Effect Sterile family member (mes-3) [Caenorhabditis elegans] | 0.047 | 13/26 (50%) | STP sterile_progeny |
| 20 | TVm01_A12 | 91 | - | 18 | No significant hits | - | - | - |
| 21 | TVm02_D11 | 86 | M | 24 | No significant hits | - | - | - |
| 22 | TVm03_G11 | 108 | - | 20 | No significant hits | - | - | - |
| 23 | TVm04_F02 | 97 | - | 19 | No significant hits | - | - | - |
| 24 | TVm06_F08 | 72 | - | 25 | No significant hits | - | - | - |
| 25 | TVm07_A07 | 78 | - | 26 | No significant hits | - | - | - |
| 26 | TVm07_G02 | 124 | - | 16 | putative calcium-binding mitochondrial carrier protein [Brugia malayi] | 1.00E-036 | 75/116 (64%) | GRO slow_growth |Unclassified |
| 27 | TVm08_D01 | 64 | M | 19 | Hypothetical protein MGG_00752 [Magnaporthe grisea 70-15] | 8.00E-006 | 25/52 (48%) | None |
| 28 | TVm08_H01 | 120 | - | 19 | Pyruvate kinase [Caenorhabditis elegans] | 6.00E-025 | 42/87 (48%) | EMB, LET embryonic_lethal |
| 29 | TvfContig1 | 152 | - | 22 | No significant hits | - | - | - |
| 30 | TvfContig3 | 143 | - | 23 | C-type LECtin family member (clec-88) [Caenorhabditis elegans] | 5.00E-021 | 55/132 (41%) | No observed phenotype is found. |
| 31 | TvfContig5 | 134 | M | 22 | Hypothetical protein K06A9.1c [Caenorhabditis elegans] | 0.004 | 28/98 (28%) | None |
| 32 | TvfContig78 | 161 | - | 20 | MSH (MutS Homolog) family member (msh-2) [Caenorhabditis elegans] | 2.00E-027 | 70/167 (41%) | spontaneous mutation rate increased |
| 33 | TvfContig84 | 104 | M | 18 | No significant hits | - | - | - |
| 34 | TvfContig88 | 145 | M | 22 | similar to TPRXL protein [Pan troglodytes] | 0.002 | 31/122 (25%) | None |
| 35 | TvfContig122 | 97 | - | 24 | No significant hits | - | - | - |
| 36 | TvfContig132 | 214 | M | 13 | Vitellogenin-6 precursor [Caenorhabditis elegans] | GRO slow_growth |EMB, LET embryonic_lethal | ||
| 37 | TVf03_C08 | 132 | - | 20 | No significant hits | - | - | - |
| 38 | TVf04_D01 | 162 | - | 16 | putative serine-threonine kinase PAR-4 [Caenorhabditis elegans] | 0.027 | 16/35 (45%) | No observed phenotype is found. |
| 39 | TVf06_A07 | 145 | - | 22 | 15 kDa excretory/secretory protein [Haemonchus contortus] | 6.00E-004 | 39/118 (33%) | No observed phenotype is found. |
| 40 | TVf07_A05 | 146 | - | 23 | No significant hits | - | - | - |