Table 3.

Comparision of annotations for T. vitrinus ESTs: EST annotations performed by Nisbet and Gasser [4] based on BLAST similarity results in first three columns and annotations obtained from ESTExplorer are shown in next columns. Example catagories selected for male ESTs are major sperm protein-like, protein kinases and protein phosphatases, transcription factors and related and nucleic acid synthesis and function and for female ESTs, vitellogenins, carbohydrate metabolism and modification, ubiquitin-proteasome pathway, protein kinases/phosphatases and transcription factors and related proteins

Manual annotation using BLAST			Annotations obtained automatically from ESTExplorer
MALE: Protein kinases and protein phosphatases
EST ID	E-value	BLAST results	BLAST results	E-value	Gene Ontologies	Metabolic Pathway Mapping	Domain/Motif data
TVm02_C07	2.00E-37	PP1-gamma serine/threonine protein phosphatase	protein phosphatasecatalyticgamma isoform isoform 1	1.00E-36	chromatin modification, protein amino acid dephosphorylation, embryonic cleavage, cytokinesis, meiosis, oviposition, manganese ion binding, protein phosphatase type 1 activity, mitochondrial outer membrane, protein binding, mitosis, glycogen metabolic process, iron ion binding, nucleus	Long-term potentiation, Regulation of actin cytoskeleton, Focal adhesion, Insulin signaling pathway	Metallophosphoesterase, Serine/threonine-specific protein phosphatase and bis(5-nucleosyl)-tetraphosphatase
TVm02_F11	2.00E-45	Serine/threonine protein kinase of the casein kinase I subfamily	tau tubulin kinase	1.00E-44	protein kinase activity, ATP binding, protein amino acid phosphorylation, locomotory behavior, growth, larval development (sensu Nematoda)	Nicotinate and nicotinamide metabolism, Inositol phosphate metabolism, Benzoate degradation via CoA ligation	Protein kinase
TVm08_H06	5.00E-60	Member of the protein phosphatase protein family	serine threonine protein phosphatase pp1	1.00E-64	phosphoprotein phosphatase activity, protein amino acid dephosphorylation, iron ion binding, manganese ion binding	None	Metallophosphoesterase, Serine/threonine-specific protein phosphatase and bis(5-nucleosyl)-tetraphosphatase
TVm09_B11	8.00E-34	Protein phosphatase-1 (PP1)	phosphoprotein phosphatase 1	1.00E-33	growth, larval development (sensu Nematoda), embryonic development ending in birth or egg hatching, hydrolase activity	None	Metallophosphoesterase, Serine/threonine-specific protein phosphatase and bis(5-nucleosyl)-tetraphosphatase
MALE: Transcription factors and related
EST ID	E-value	BLAST results	BLAST results	E-value	Gene Ontologies	Metabolic Pathway Mapping	Domain/Motif data
TVm01_F11	8.00E-10	Transcription elongation factor (testes specific homologue in mouse)	transcription elongation factor a1	1.00E-19	regulation of transcription, DNA-dependent, RNA elongation, translation elongation factor activity, zinc ion binding, RNA polymerase II transcription factor activity, nucleus, DNA binding, defense response, transcription regulator activity, transcription, regulation of transcription, transcription factor activity, transcription elongation factor complex, protein binding, transcriptional elongation regulator activity, erythrocyte differentiation, positive regulation of transcription, DNA-dependent, general RNA polymerase II transcription factor activity, metal ion binding, nucleoplasm	None	None
TVm09_B03	1.00E-09	Protein containing six kelch motifs and one BTB/POZ domain	klhl10 protein	1.00E-10	larval development (sensu Nematoda), growth, protein binding	None	Kelch repeat
FEMALE: Vitellogenins
EST ID	E-value	BLAST results	BLAST results	E-value	Gene Ontologies	Metabolic Pathway Mapping	Domain/Motif data
TVf01_C08	4.00E-25	Vitellogenin 6 precursor	vitellogenin structural genes (yolk protein genes) family member (vit-6)	1.00E-24	embryonic development ending in birth or egg hatching;P:determination of adult life span, lipid transporter activity	None	Lipid transport protein, N-terminal
TVf01_C11	7.00E-14	Vitellogenin 5 precursor	vitellogenin structural genes (yolk protein genes) family member (vit-6)	1.00E-25	embryonic development ending in birth or egg hatching;P:determination of adult life span, lipid transporter activity	None	Lipid transport protein, N-terminal
FEMALE: Carbohydrate metabolism and modification
EST ID	E-value	BLAST results	BLAST results	E-value	Gene Ontologies	Metabolic Pathway Mapping	Domain/Motif data
TVf02_G06	7.70E-15	glyceraldehyde 3 phosphate dehydrogenase gpd-3C	glyceraldehyde-3-phosphate dehydrogenase	1.00E-18	cell wall chitin biosynthetic process, glutamine-fructose-6-phosphate transaminase (isomerizing) activity	None	Glyceraldehyde 3-phosphate dehydrogenase, Lipocalin
TVf02_H12	6.80E-24	glucosamine-fructose-6-phosphate aminotransferase	glutamine-fructose-6-phosphate transaminase 2	1.00E-32	fructose 6-phosphate metabolic process, glutamine-fructose-6-phosphate transaminase (isomerizing) activity	Aminosugars metabolism, Glutamate metabolism	Glutamine amidotransferase, class-II
TVf03_B07	5.80E-28	Pyruvate dehydrogenase alpha subunit	pyruvate dehydrogenase e1 alpha subunit	1.00E-62	oxidoreductase activity, acting on the aldehyde or oxo group of donors, disulfide as acceptor, metabolic process, C:mitochondrion	Pyruvate metabolism, Glycolysis/Gluconeogenesis, Valine leucine and isoleucine biosynthesis, Butanoate metabolism	Dehydrogenase, E1 component
TVf03_E01	2.80E-35	Pyruvate dehydrogenase	dihydrolipoamide acetyltransferase	1.00E-53	oxidoreductase activity	Pyruvate metabolism, Glycolysis/Gluconeogenesis, Valine leucine and isoleucine biosynthesis, Butanoate metabolism	Transketolase, central region
TVf06_E11	3.80E-49	gei-7 isocitrate lyase	malate synthase a	1.00E-50	embryonic development, F:acyltransferase activity, tricarboxylic acid cycle, isocitrate lyase activity, determination of adult life span, glyoxylate cycle	Pyruvate metabolism, Glyoxylate and dicarboxylate metabolism	Malate synthase
TVf09_D01	3.20E-08	Ribulose-phosphate 3 epimerase	ribulose-phosphate 3-epimerase	1.00E-17	metabolic process, catalytic activity	Pentose phosphate pathway	Ribulose-phosphate 3-epimerase
FEMALE: Ubiquitin-proteasome pathway
EST ID	E-value	BLAST results	BLAST results	E-value	Gene Ontologies	Metabolic Pathway Mapping	Domain/Motif data
TVf02_H07	3.00E-28	Ubiquitin-like	ubiquitin family member (ubq-1)	1.00E-27	ribosome, larval development (sensu Nematoda), structural constituent of ribosome, reproduction, growth translation, protein modification process	Ribosome	Ubiquitin
TVf06_D11	1.30E-12	pbs-2 Proteasome A-type and B-type	20s proteasome beta subunit pbb2	1.00E-22	threonine endopeptidase activity, ubiquitin-dependent protein catabolism, proteasome core complex (sensu Eukaryota)	Proteasome	20S proteasome, A and B subunits
TVf10_F06	5.20E-07	ubiquitin activating enzyme related (uba-1)	ubiquitin-activating enzyme e1	1.00E-12	ATP binding, ubiquitin-protein ligase activity, ubiquitin activating enzyme activity	Ubiquitin mediated proteolysis	Ubiquitin-activating enzyme repeat
FEMALE: Protein kinases/phosphatases
EST ID	E-value	BLAST results	BLAST results	E-value	Gene Ontologies	Metabolic Pathway Mapping	Domain/Motif data
TVf06_F11	1.40E-49	lin-2 erythrocyte membrane like protein	calcium calmodulin-dependent serine protein kinase (maguk family)	1.00E-51	synapse, guanylate kinase activity, cell adhesion, positive regulation of vulval development (sensu Nematoda), actin cytoskeleton, protein-tyrosine kinase activity, synaptosome, nucleotide binding, basolateral plasma membrane, protein amino acid phosphorylation, cytosol, cell junction, calmodulin binding, protein serine/threonine kinase activity, positive regulation of transcription from RNA polymerase II promoter	Tight junction, Neurodegenerative Disorders	Pkinase_Tyr, Guanylate_kin
FEMALE: Transcription factors and related
EST ID	E-value	BLAST results	BLAST results	E-value	Gene Ontologies	Metabolic Pathway Mapping	Domain/Motif data
TVf01_C02	3.80E-18	ama-1 RNA polymerase II	rna polymerase ii largest subunit	1.00E-30	DNA-directed RNA polymerase activity, DNA binding, transcription from RNA polymerase II promoter, DNA-directed RNA polymerase II, core complex	Purine metabolism, Pyrimidine metabolism, RNA polymerase	RNA polymerase Rpb1, domain 1
TVf03_A03	9.70E-25	btf-1 helicase	tbp associated factor	1.00E-63	nucleic acid binding, helicase activity, ATP binding	Purine metabolism	Helicase, C-terminal
TVf04_E07	2.00E-09	ceh-5 Homeobox domain	similarity to lim-homeobox protein lmx1_mesau	1.00E-15	metal ion binding, sequence-specific DNA binding, transcription factor activity, regulation of transcription, multicellular organismal development DNA-dependent, nucleus	None	None