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. 2008 Mar;10(3):217–222. doi: 10.1593/neo.07945

Figure 1.

Figure 1

Effect of siRNA-mediated reduction of α-actinin-1 on pressure-stimulated murine colon cancer cell adhesion to collagen I. (A) Typical reduction of α-actinin-1 protein in CT26 cells transfected with siRNA targeted to α-actinin-1 (siACTN1) compared with cells transfected with a nontargeting sequence (siNT) as measured by Western blot analysis. Glyceraldehyde-3-phosphate dehydrogenase was used as a loading control, and α-actinin-1 protein expression is normalized against that of siNT-transfected cells (*P < .01; n = 11). (B) The 15 mm Hg-increased extracellular pressure (closed bars) stimulates siNT-transfected CT26 cell adhesion to collagen I compared with ambient pressure controls (open bars). CT26 cells transfected with siACTN1 fail to display any increase in adhesion due to pressure (*P < .04; n = 6; #P < .04 comparison of siNT versus siACTN1 adhesion under ambient pressure). Data from individual experiments were normalized to the siNT ambient pressure controls and graphically expressed as mean ± SEM. Statistical analysis was by Wilcoxon matched-pairs signed-ranks test.