Figure 4.

Effect of increased pressure and α-actinin-1 reduction on CT26 cell proliferation. CT26 cells were transfected with either siACTN1 or siNT and, after 48 hours, were exposed to either 15 mm Hg-increased pressure or ambient pressure conditions. After exposure to pressure (time 0), cell proliferation of the respective populations was assessed by colorimetric analysis of MTT reduction every 24 hours. No significant difference in the rate of proliferation was observed between experimental groups (n = 4). All data are represented as mean ± SEM.