Figure 1.

Agarose gel electrophoresis showing amplifiers of different secretory phospholipase A₂ isozymes (sPLA₂-IIA, sPLA₂-V, and sPLA₂-X) and GAPDH mRNA after RT-PCR analysis expressed in normal and malignant prostate cell lines under basal conditions and after exposure to proinflammatory cytokines. Cells were incubated for 24 hours in medium containing 10% FCS alone and additions of cytokines at a final concentration of 25 ng/ml. Lanes 1 to 5 are controls (without additions of cytokines), IFN-γ, IL-1β, IL-6, and TNF-α, respectively. Data are representative of at least three independent experiments giving similar results. AD and TP indicate the androgen dependence and tumorigenicity potential according to literature [32,34].