Figure 1.

Formation of DMPO/^⋅OH and DEPMPO/^⋅OH adducts during the reaction between H₂O₂ and WT-SOD or SOD mutants. (A) H₂O₂ (5 mM), DMPO (50 mM), DTPA (100 μ M), and WT-SOD (100 μg/ml) or A4V or G93A (100 μg/ml) were incubated in bicarbonate/CO₂ buffer (25 mM, pH 7.4). (B) H₂O₂ (5 mM), DEPMPO (50 mM), DTPA (100 μM), and WT-SOD (100 μg/ml) or A4V or G93A (100 μg/ml) were incubated in bicarbonate/CO₂ buffer (25 mM, pH 7.4). The spectra were recorded immediately after the reaction was initiated with the enzyme. Spectra are representative scans of three independent experiments.