Table 2.
The relative concentrations of 17O and 16O spin adducts of DEPMPO/⋅OH formed during the reaction of SOD with 89% [17O]-H2O2
| Conditions | % of DEPMPO/⋅OH spin adducts from H2O2 | % of DEPMPO/⋅OH spin adducts from H2O |
|---|---|---|
| Fe(II)-EDTA | 100 | 0 |
| WT-SOD | 60 ± 2.82 | 40 ± 2.82 |
| A4V | 66 ± 0.47 | 34 ± 0.47 |
| G93A | 63 ± 0.47 | 37 ± 0.82 |
Data were normalized with respect to the 17O content in H217O2 by using the formula: %DEMPMPO/⋅OH from water = 100(%H217O2 − %DEPMPO/⋅17OH)/%H217O2.