Table 1.
Strain | Genotype |
---|---|
MAY200 | MATa ade2-101 his3-Δ201 lys2-801 trp1-Δ901 ura3-52 (hht1 hhf1)::LEU2 (hht2 hhf2)::HIS3 plus pRM200 (CEN4 ARS1 TRP1 HHT2-HHF2) and pRM102 (CEN4 ARS1 URA3 pGAL10-HHT2 pGAL1-HHF2) |
RMY430 | MATa ade2-101 his3-Δ201 lys2-801 trp1-Δ901 ura3-52 (hht1 hhf1)::LEU2 (hht2 hhf2)::HIS3 plus pRM430 (CEN4 ARS1 TRP1 hht2-Δ430 HHF2) |
MAY430 | Same as RMY430, except pMX430 (hht2-Δ430 HHF2 cloned into pRS317) in place of pRM430, plus pRM102 (CEN4 ARS1 URA3 pGAL10-HHT2 pGAL1-HHF2) |
MAY817 | Same as MAY430, except pMX817 (hht2-Δ430, hhf2-Δ414) |
MAY818 | Same as MAY430, except pMX818 (hht2-Δ430, hhf2-Δ419) |
MAY502 | Same as MAY430, except pMX502 (hht2-Δ430, hhf2-K5,8,12G) |
MAY503 | Same as MAY430, except pMX503 (hht2-Δ430, hhf2-K5,8,12,16G) |
MAY504 | Same as MAY430, except pMX504 (hht2-Δ430, hhf2-K8, 12, 16G) |
MAY505 | Same as MAY430, except pMX505 (hht2-Δ430, hhf2-K5,12,16G) |
MAY506 | Same as MAY430, except pMX506 (hht2-Δ430, hhf2-K5,8,16G) |
MAY512G | Same as RMY430, except pK512G (hht2-Δ430, hhf2-K5, 12G) |
MAY512R | Same as RMY430, except pK512G (hht2-Δ430, hhf2-K5, 12R) |
MAY58R | Same as RMY430, except pK58R (hh2-Δ430, hhf2-K5, 8R) |