Figure 2.

Rpp2 protein coimmunoprecipitates with the Rpp1 protein and the RNase P and RNase MRP RNAs. (A) Immunoprecipitates derived from wild-type (untagged) cells (VS211B, lane 1), FLAGHISRPP2 and 3xmycRPP1 double epitope-tagged cells (VS203, lane 2), and FLAGHISRPP2-tagged cells (VS202A, lane 3) were denatured, resolved on a 12% SDS/PAGE gel, transferred to nitrocellulose membranes, and immunoblotted with polyclonal antibodies against the myc and his epitopes. (B) Immunoprecipitated RNAs extracted from equivalent amounts of anti-flag Ab-IgG-agarose beads that were incubated with equivalent amounts of cell extracts from FLAGHISRPP2-tagged cells (VS202A, lane 1) and wild-type (untagged) cells (VS211B, lane 2) were 3′ end-labeled with [5′-³²P]pCp and separated on an 8% polyacrylamide/7 M urea gel. The upper band is RNase P RNA (RPR1), and the lower band is RNase MRP RNA (NME1). tRNA species are found nonspecifically in both immunoprecipitates.