Figure 2.

Crosslinking of pc to K23Q–PsaF is strongly impaired. Immunoblot analysis of crosslinked products between pc or cyt c₆ and PSI I from wild-type (lanes 1–3, 2 μg Chl loaded on each lane), K23Q transformant (lanes 4–6, 3 μg chl loaded on each lane), K12P transformant (lanes 7–9, 2 μg Chl loaded on each lane), K16Q transformant (lanes 10–12, 2 μg Chl loaded on each lane), and K30Q transformant (lanes 13–15, 2 μg Chl loaded on each lane) fractionated by SDS/PAGE. The blot was probed with anti-PsaF antibodies. Pc or cyt c₆ (20 μM) were used for the crosslinking experiments.