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. 1998 Jun 9;95(12):6722–6727. doi: 10.1073/pnas.95.12.6722

Figure 1.

Figure 1

The TFIIH-independent function of TFIIE. (A) The effect of TFIIE in basal transcription was determined in the absence of TFIIH as described in Materials and Methods, by using the templates indicated. Transcription was in the absence (lanes 1 and 3) or presence of recombinant TFIIE (lane 2; 2 ng, and lane 4; 4 ng each of E56/E34) or mutant (mt) TFIIE (lane 5; 4 ng each of E34 and mt E56; ref. 13). Primer extension products of the transcripts are indicated by an arrowhead (G6TI) and a bracket (E4). (B) The effect of TFIIE on TBP binding to AdML 2 and E4 promoter DNA in DNase I-footprinting analyses on the nontranscribed DNA strand (lanes 1–4 and 11–13) and the transcribed DNA strand (lanes 5–10). Proteins in the binding reactions are indicated on the top, including “no protein” (lanes 4, 5, and 11). Ten nanograms each of TBP and TFIIA and 8 ng each of E56 and E34 were used. Nucleotide positions on the promoter relative to the start site of transcription of the AdML 2 promoter are numbered, and protected regions against DNase I digestion over the TATA box are indicated by brackets. The protection indicated by an asterisk in lane 8 corresponds to the cryptic TATA sequence further upstream of the TATA box. TATA box (“TATAAAA”) is indicated by a bar.