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Requirement for the transposase binding sites in transposition. (A) Structure of the 2Km2–0Km0 series of Tn552kan elements (details are identical to those in Fig. 2). (B) Transposition reactions (2 hr) contained 10 mM Mg2+, 225 ng of supercoiled pUC19, and about 18 fmol of 32P-labeled substrate as indicated. Samples in even-numbered lanes were digested with PstI. Product analysis was as before.
