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Effects of glycerol and NaCl on strand transfer activity. Reactions (1 hr) contained 180 ng (Left) or 270 ng (Right) of supercoiled pUC19 as target, about 50 fmol of 32P-labeled 2Km2 as substrate, and 10 mM Mg2+. Concentrations of NaCl and glycerol were as indicated. Product analysis was as before.
