Figure 2.

Distribution of the backbone amide chemical shift changes within AIRE–PHD1 on binding to H3 amino-terminal peptides. Histograms showing the average backbone chemical shift differences observed in the ¹⁵N-labelled AIRE–PHD1 (0.2 mM) on addition of a twofold excess of (A) H3K4me0, (B) H3K4me1, (C) H3K4me2 and (D) H3K4me3; the average chemical shift differences decrease with the methylation level of H3K4. Asterisks indicate residues for which the backbone amide signals disappear during titration owing to line broadening. AIRE, autoimmune regulator; H3K4me0, histone H3 non-methylated at lysine 4; H3K4me1 (H3K4me2, H3K4me3), histone H3 monomethylated (respectively, dimethylated and trimethylated) at lysine 4; PHD, plant homeodomain.