Table 1.

Values of the dissociation constants between H3 peptides and AIRE–PHD1 wild type (WT) and mutants measured by fluorescence spectroscopy and isothermal titration calorimetry

AIRE–PHD1	Peptide	KD (μM), fluorescence spectroscopy	KD (μM), isothermal titration calorimetry
WT	H3K4me0	4.7±0.8	6.5±0.2
WT	H3K4me1	21.4±5.9	55.6±1.2
WT	H3K4me2	>500	714±90
WT	H3K4me3	ND	NM
V301M	H3K4me0	6.8±0.4	NM
C311Y	H3K4me0	ND	NM
D297A	H3K4me0	173.0±18.6	146.8±6.1
D312A	H3K4me0	ND	ND
WT	H3K4me0-R2A	ND	NM
D297A	H3K4me3	ND	NM
ND, not detectable, denotes binding too weak to be reliably quantified; NM, not measured.