Figure 3.

Expansion and dissemination of CD3⁻CD4⁻CD8⁺ T cell progenitors in the Blk(Y495F)-6 transgenic line. Single-cell suspensions were made from thymus, spleen, lymph nodes, and bone marrow from thymomatous Blk(Y495)-6 transgenic mice or from age-matched, nontransgenic littermates. Cells were stained, singly or in combination, with PE- (y axis) and FITC- (x axis) conjugated antibodies to Thy1.2, CD3, αβ TCR, CD8, CD4, CD44, CD25, CD19, and B220 as indicated. The founder, four transgenic progeny, and three normal mice were examined; representative results are presented.