Figure 2.

The levels of Holliday junctions in ρ⁻ mtDNAs are independent of Abf2p. (A) 2D gel electrophoresis of replication and recombination intermediates from the 2-kb VAR1 petite, 2–33, in ABF2 (Upper) and Δabf2 (Lower) 14WW cells. The blots were hybridized with a probe specific for petite mtDNA. The arrows indicate high molecular weight species, which may be representative of single-strand tailing resulting from rolling circle replication. (B) One-dimensional gel electrophoresis of ρ⁻ 2–33 mtDNA linearized with increasing concentrations of HincII (0.25, 0.5, and 1 unit/μl) from ABF2 and Δabf2 14WW cells. Molecular size markers and the expected migration positions of linear double-stranded and Holliday junction DNAs are indicated.