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Effect of ABF2 and MGT1 alleles on the stability of ρ+ mtDNA. The kinetics of petite induction were determined for ABF2 MGT1 (○), Δabf2 MGT1 (□), ABF2 Δmgt1 (▴), and Δabf2 Δmgt1 (⧫) 14WW cells. Cells were shifted from YPG to YPD medium, and aliquots were plated at various intervals and on YPD medium. The fraction of ρ+ colonies was scored by overlaying with 2,3,5-triphenyl tetrazolium chloride soft agar as described in Materials and Methods.
