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. 2008 Jan 24;27(4):654–666. doi: 10.1038/emboj.2008.1

Figure 1.

Figure 1

KSHV genome-wide ChIP analysis: colocalization of CTCF and cohesins upstream of ORF73. (A) A 384-well array of PCR primers spanning ∼400-bp intervals across the KSHV genome was used to analyse ChIP DNA isolated with antibodies specific for LANA, ORC2, CTCF, SMC1, SMC3, RAD21, or control IgG. The KSHV coordinates are indicated below each column of graphs. •LANA- and ORC2-binding sites at TR; *location of the major CTCF–cohesin-binding site upstream of ORF73. (B) Schematic of the region of KSHV genome (nt 116 000–138 000) containing the major latency transcript (leftward ORF73/72/71, vmiRNA, and K12), the rightward-oriented K14/ORF74 lytic transcript, and the TR. (C) Western blot of BCBL1 cell extracts with antibodies to CTCF, SMC1, SMC3, and RAD21 used for ChIP in (A). (D) Co-immunoprecipitation assay with antibodies to CTCF, SMC1, RAD21, or IgG control. IPs were immunoblotted with anti-CTCF.