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Melting temperatures and hyperchromicity determined by UV absorbance for dodecamer DNA duplexes containing 5-OHU:X base pairs (where X= A, C, G, or T)
| Duplex | tm(°C) | %hyperchromicity |
|---|---|---|
| 5-OHU:A | 50.3 | 16 |
| 5-OHU:C | 40.7 | 2 |
| 5-OHU:G | 53.7 | 14 |
| 5-OHU:T | 46.6 | 9 |
