Table 1.

Summary of sec26 mutants and genetic interactions with GLO3

Name	Mutations	Restrictive temperature1	GLO3 suppression?	glo3Δ synthetic lethality?
FW	F856A W860A	40°	YES	YES
TDD	T536A D903A D904A (sec26-1)	34°	YES	YES
7A4	K659R I681V D759V S810P C905S I938V L950S	37°	YES	YES
7D15	S725Y K732N N772I H774R F777Y F799I K915M K964T	37°	YES	YES
9C15	C678S F693S D759G Y823C V838A I842T Y913H	37°	YES	YES
10D22	R672G I727T V743G K807R T877A	37°	YES	YES
10D25	L651Q K659E H839P I846T F863I A959S	37–40°	YES	YES
11A3	K659E K679N I733L Y744C F754S Y832N A851V F863L I893T I938L V973A	37°	YES	YES
12B6	N680T R691W D741G F817Y M859V	37–40°	YES	YES
1A11	F856A W860A D904A C905S	37–40	YES	+/-
11D26	E633D I733T D741V T769P F802L (sec26-2)	37°	YES	+/-
12D18	Q661R S740P V806A F907Y F918S	37°	YES	+/-
11C15	S628P I785V M859I L950S D956V H972Y	37°	YES	+/-
7D20	S740P I785T N793S K801E F863S A951T	37°	YES	no
9A1	I598N S728T D759V D823G I846T H855L I869L G901R	37°	YES	no
10D24	V623A S628T P636A V757A T779S W860R K887R T895A K933E A962T	37°	YES	no
11B10	L773Q I795T H839L N966Y	37°	YES	no
11C13	Q607L M624V L646P H670P C678S I846T T877I N890S E920V K963E	34°	YES	no
9C16	S626P K655R N793H T803S G818S N836D K868R E929K Q948L K963R	37–40°	YES	no
5A7	E633G N635Y E663G K666R F690L R691W S728L V756D Q776R D921G S932T L950M	34°	no	YES
7C13	F650L G695S H797Q I821N W860L E902G	34°	no	YES
7D17	K655N A665T D676G Q702R Q776H G781C Y822N D844E F863Y R883S	37°	no	YES
7D18	K614R L780H V794E A825V L885M C910R C927S A962S	37°	no	YES
9A2	Q607R K669M I846F M859V F907L F918Y	40°	no	YES
9C14	D697N D704H S740T I820T R831C I842V I928F S932T	37°	no	YES
10C20	S608T D616E D676G F693L D704G S726G P735H E746K H774Y F799L K847I F856Y	34°	no	YES
10D26	I598T R622G A718P T722I P735L K767E H839P T850S N890S D921E N966Y T971A	37°	no	YES
11B11	I615F L646Q K715R F817S F863L K872E M891L L950S	34–37°	no	YES
11C14	R622G K679G A694D A924V C927S V937D	40°	no	YES
11D30	A649E C910R S917P	37°	no	YES
11C20	D676N L730R F754L N772Y A830D I834N D852V S917T F918I	37°	no	YES
7A5	S619F L620V D709E R957G	40°	no	+/-
11C19	V600D F658S L712Q Q753H Q776R N836I T965A	37°	no	+/-
7D16	S628T L712Q P789S V794E D829V I846F I961F2	34–37°	no	no
11B8	I615F R660G S724C S916P K964E L970H	40°	no	no
11B9	E617V I629S K715I T889S A924V A959H3	34°	no	no
11C16	I662F V701G H720P V743G K784R F856I K887E	40°	no	no
11C17	L620I M624I L646P T654S T686M I749V V794G N867S K868G	37°	no	no
11D25	S628P A718S I733M P796T A825V Y832F3	37–40°	no	no
12C13	S628P G798C F802S E866G Y913C	34°	no	no

¹ restrictive temperature on SCGalactose media

² also contains additional residues at COOH-terminus ⁹⁷⁴WTTLS⁹⁷⁸

³ also contains deletion of COOH-terminal residues 960–973